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KMID : 0358320090500050432
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Korean Journal of Urology
2009 Volume.50 No. 5 p.432 ~ p.438
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The Relationship between RUNX3 Inactivation and Its Pathological Features in Renal Cell Carcinoma
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Kwon Whi-An
Park Cheol
Kim Eun-Jung
Ha Yun-Sok
Kim Yong-June
Yun Seok-Joong
Lee Sang-Cheol
Kim Wun-Jae
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Abstract
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Purpose: DNA methylation is a key regulator of gene transcription and genomic stability, and alterations in DNA methylation are frequently detected in human tumors. Recent study has suggested that inactivation of runt-related transcription factor 3 (RUNX3), primarily epigenetic alterations in DNA methylation, is closely associated with bladder tumor stage, grade, and prognosis. The aim of this study was to evaluate the association between RUNX3 inactivation and renal cell carcinoma (RCC).
Materials and Methods: RCC tissues (n=56) were obtained from patients who underwent radical nephrectomy. The methylation pattern of RUNX3 was determined by using methylation specific-polymerase chain reaction (MS-PCR) and direct DNA sequencing.
Results: Methylation of the RUNX3 promoter was observed in 75.0% of the samples (42/56). The tumor stage and grade were significantly associated with the methylation status (p＜0.05, respectively). However, recurrence and progression of RCC were not significantly related to the methylation of the RUNX3 promoter region (log-rank test, p＞0.05, respectively).
Conclusions: This study demonstrated that promoter methylation of RUNX3 is frequently observed in RCC. In addition, RUNX3 methylation is closely associated with aggressive pathologic features.
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KEYWORD
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Human RUNX3 protein, Methylation, Renal cell carcinoma
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